High throughput screening and identification of coagulopathic snake venom proteins and peptides using nanofractionation and proteomics approaches.

ABSTRACT: Snakebite is a neglected tropical disease that results in a variety of systemic and local pathologies in envenomed victims and is responsible for around 138,000 deaths every year. Many snake venoms cause severe coagulopathy that makes victims vulnerable to suffering life-threating haemorrhage. The mechanisms of action of coagulopathic snake venom toxins are diverse and can result in both anticoagulant and procoagulant effects. However, because snake venoms consist of a mixture of numerous protein and peptide components, high throughput characterizations of specific target bioactives is challenging. In this study, we applied a combination of analytical and pharmacological methods to identify snake venom toxins from a wide diversity of snake species that perturb coagulation. To do so, we use

SUBMITTER: Slagboom J 

PROVIDER: S-EPMC7153897 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature