Determinants of Replication-Fork Pausing at tRNA Genes in Saccharomyces cerevisiae.

ABSTRACT: Transfer RNA (tRNA) genes are widely studied sites of replication-fork pausing and genome instability in the budding yeast Saccharomyces cerevisiae tRNAs are extremely highly transcribed and serve as constitutive condensin binding sites. tRNA transcription by RNA polymerase III has previously been identified as stimulating replication-fork pausing at tRNA genes, but the nature of the block to replication has not been incontrovertibly demonstrated. Here, we describe a systematic, genome-wide analysis of the contributions of candidates to replication-fork progression at tDNAs in yeast: transcription factor binding, transcription, topoisomerase activity, condensin-mediated clustering, and Rad18-dependent DNA repair. We show that an asymmetric block to replication is maintained even whe

SUBMITTER: Yeung R 

PROVIDER: S-EPMC7153945 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature