MiR-138 Increases Depressive-Like Behaviors by Targeting SIRT1 in Hippocampus.

ABSTRACT:

Background

Major depressive disorder (MDD) is a serious and common mood disorder with unknown etiology. Emerging evidence has demonstrated the critical roles of SIRT1 and microRNAs (miRNAs) in the progression of MDD. However, the underlying molecular mechanisms remain to be fully understood.

Methods

In the present study, the expression level of miR-138 and SIRT1 were analyzed by RT-PCR or Western blotting in a chronic unpredictable mild stress (CUMS) model. The depressive-like behaviors were analyzed by forced swimming test (FST) and sucrose preference test (SPT) in mice injected with miR-138 and SIRT1 overexpression lentivirus. The luciferase reporter assay was used to assess the direct regulation of miR-138 on SIRT1 expression.

Results

The upregulation of miR-138 wa

SUBMITTER: Li C 

PROVIDER: S-EPMC7154038 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature