Imaging the Cell Morphological Response to 3D Topography and Curvature in Engineered Intestinal Tissues.

ABSTRACT: While conventional cell culture methodologies have relied on flat, two-dimensional cell monolayers, three-dimensional engineered tissues are becoming increasingly popular. Often, engineered tissues can mimic the complex architecture of native tissues, leading to advancements in reproducing physiological functional properties. In particular, engineered intestinal tissues often use hydrogels to mimic villi structures. These finger-like protrusions of a few hundred microns in height have a well-defined topography and curvature. Here, we examined the cell morphological response to these villus-like microstructures at single-cell resolution using a novel embedding method that allows for the histological processing of these delicate hydrogel structures. We demonstrated that by using photopolymer

SUBMITTER: Altay G 

PROVIDER: S-EPMC7154059 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature