Biomarker Signatures of Quality for Engineering Nasal Chondrocyte-Derived Cartilage.

ABSTRACT: The definition of quality controls for cell therapy and engineered product manufacturing processes is critical for safe, effective, and standardized clinical implementation. Using the example context of cartilage grafts engineered from autologous nasal chondrocytes, currently used for articular cartilage repair in a phase II clinical trial, we outlined how gene expression patterns and generalized linear models can be introduced to define molecular signatures of identity, purity, and potency. We first verified that cells from the biopsied nasal cartilage can be contaminated by cells from a neighboring tissue, namely perichondrial cells, and discovered that they cannot deposit cartilaginous matrix. Differential analysis of gene expression enabled the definition of identity markers for the tw

SUBMITTER: Asnaghi MA 

PROVIDER: S-EPMC7154140 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature