Cloning, tissue distribution, expression pattern, and function of porcine maternal embryonic leucine zipper kinase.

ABSTRACT:

Background

Maternal embryonic leucine zipper kinase (MELK) is an atypical member of the snf1/AMPK family of serine-threonine kinases, involved in diverse physiological and pathological processes, including cell proliferation, apoptosis, embryogenesis, cancer treatment resistance, and RNA processing. MELK is highly expressed in human cancers and is associated with more aggressive forms of astrocytoma, glioblastoma, breast cancer, and melanoma to date, no information about porcine MELK (pMELK) has been reported.

Methods

In this study, the pMELK coding sequence was cloned from swine spleen and characterized. We also quantitatively determined the expression of MELK in 11 tissues isolated from a piglet and determined its subcellular localization when expresse

SUBMITTER: Chen P 

PROVIDER: S-EPMC7154462 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature