Use of an Outbred Rat Hepacivirus Challenge Model for Design and Evaluation of Efficacy of Different Immunization Strategies for Hepatitis C Virus.

ABSTRACT:

Background and aims

The lack of immunocompetent small animal models for hepatitis C virus (HCV) has greatly hindered the development of effective vaccines. Using rodent hepacivirus (RHV), a homolog of HCV that shares many characteristics of HCV infection, we report the development and application of an RHV outbred rat model for HCV vaccine development.

Approach and results

Simian adenovirus (ChAdOx1) encoding a genetic immune enhancer (truncated shark class II invariant chain) fused to the nonstructural (NS) proteins NS3-NS5B from RHV (ChAd-NS) was used to vaccinate Sprague-Dawley rats, resulting in high levels of cluster of differentiation 8-positive (CD8⁺ ) T-cell responses. Following RHV challenge (using 10 or 100 times the minimum infectious dose), 42% of vacc

SUBMITTER: Atcheson E 

PROVIDER: S-EPMC7154631 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature