High-Level Expression, Purification and Initial Characterization of Recombinant Arabidopsis Histidine Kinase AHK1.

ABSTRACT: Plants employ a number of phosphorylation cascades in response to a wide range of environmental stimuli. Previous studies in Arabidopsis and yeast indicate that histidine kinase AHK1 is a positive regulator of drought and osmotic stress responses. Based on these studies AHK1 was proposed a plant osmosensor, although the molecular basis of plant osmosensing still remains unknown. To understand the molecular role and signaling mechanism of AHK1 in osmotic stress, we have expressed and purified full-length AHK1 from Arabidopsis in a bacterial host to allow for studies on the isolated transmembrane receptor. Purification of the recombinant protein solubilized from the host membranes was achieved in a single step by metal-affinity chromatography. Analysis of the purified AHK1 by SDS-polyacrylam

SUBMITTER: Hofmann A 

PROVIDER: S-EPMC7154865 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature