Human endothelial cells size-select their secretory granules for exocytosis to modulate their functional output.

ABSTRACT:

Background

The secretory granules of endothelial cells, Weibel-Palade bodies, are released in response to numerous extracellular signals. Their cargo is critical to many vascular functions including hemostasis and inflammation. This presents a fundamental problem: how can these cells initiate tailor-made responses from the release of a single type of organelle, each with similar cargo? Each cell contains Weibel-Palade bodies in a wide range of sizes, and we have shown that experimentally shortening these organelles disproportionately reduces their ability to initiate hemostasis in vitro, leaving leukocyte recruitment unaffected. Could the production of this range of sizes underpin differential responses?

Objectives

To determine whether different agonists drive the exocytosis

SUBMITTER: McCormack JJ 

PROVIDER: S-EPMC7155122 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature