Immunogenic potential of human bone marrow mesenchymal stromal cells is enhanced by hyperthermia.

ABSTRACT:

Background aims

Bone marrow-derived mesenchymal stromal cells (MSCs) have been reported to suppress T-cell proliferation and used to alleviate the symptoms of graft-versus-host disease (GVHD). MSCs are a mixed cell population and at this time there are no tools to isolate the cells responsible for the T-cell suppression. We wanted to find a way to enhance the immune-modulatory actions of MSCs and tried varying the temperature at which they were cultured.

Methods

We cultured human MSCs derived from healthy volunteers at different temperatures and tested their ability to switch macrophage character from pro-inflammatory to anti-inflammatory (M1 type to M2 type). Using an enzyme-linked immunosorbent assay (ELISA), we showed that when MSCs are cultured at higher temperatures the

SUBMITTER: McClain-Caldwell I 

PROVIDER: S-EPMC7155186 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature