Molecular Mechanisms of Class B GPCR Activation: Insights from Adrenomedullin Receptors.

ABSTRACT: Adrenomedullin (AM) is a 52 amino acid peptide that plays a regulatory role in the vasculature. Receptors for AM comprise the class B G protein-coupled receptor, the calcitonin-like receptor (CLR), in complex with one of three receptor activity-modifying proteins (RAMPs). The C-terminus of AM is involved in binding to the extracellular domain of the receptor, while the N-terminus is proposed to interact with the juxtamembranous portion of the receptor to activate signaling. There is currently limited information on the molecular determinants involved in AM signaling, thus we set out to define the importance of the AM N-terminus through five signaling pathways (cAMP production, ERK phosphorylation, CREB phosphorylation, Akt phosphorylation, and IP₁ production). We characterized t

SUBMITTER: Garelja ML 

PROVIDER: S-EPMC7155197 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature