Orotic acid-treated hepatocellular carcinoma cells resist steatosis by modification of fatty acid metabolism.

ABSTRACT:

Background

Orotic acid (OA) has been intensively utilized to induce fatty liver in rats. Although the capacity of OA to cause steatosis is species-specific, previous in vitro studies indicate that humans could also be susceptible to OA-induced fatty liver. The aim of the present study was to re-elucidate the potential of OA exposure to modulate the cellular mechanisms involved in both non-alcoholic fatty liver disease pathogenesis and cellular protection from lipid accumulation. In addition, alterations in detailed fatty acid (FA) profiles of cells and culture media were analyzed to assess the significance of lipid metabolism in these phenomena.

Methods

In our experiments, human hepatocellular carcinoma HepG2 cells were exposed to OA. Bacterial endotoxin, lipopolysaccharide

SUBMITTER: Matilainen J 

PROVIDER: S-EPMC7155272 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature