Improving production of Streptomyces griseus trypsin for enzymatic processing of insulin precursor.

ABSTRACT:

Background

Trypsin has many applications in food and pharmaceutical manufacturing. Although commercial trypsin is usually extracted from porcine pancreas, this source carries the risks of infectivity and immunogenicity. Microbial Streptomyces griseus trypsin (SGT) is a prime alternative because it possesses efficient hydrolysis activity without such risks. However, the remarkable hydrolysis efficiency of SGT causes autolysis, and five autolysis sites, R21, R32, K122, R153, and R201, were identified from its autolysate.

Results

The tbcf (K101A, R201V) mutant was screened by a directed selection approach for improved activity in flask culture (60.85 ± 3.42 U mL^-1, increased 1.5-fold). From the molecular dynamics simulation, in the K101A/R201V mutant the distance bet

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7155311 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature