Sustained neuronal and microglial alterations are associated with diverse neurobehavioral dysfunction long after experimental brain injury.

ABSTRACT: Traumatic brain injury (TBI) can cause progressive neurodegeneration, sustained neuroinflammation and chronic neurological dysfunction. Few experimental studies have explored the long-term neurobehavioral and functional cellular changes beyond several months. The present study examined the effects of a single moderate-level TBI on functional outcome 8 months after injury. Male C57BL/6 mice were subjected to controlled cortical impact injury and followed for changes in motor performance, learning and memory, as well as depressive-like and social behavior. We also used a novel flow cytometry approach to assess cellular functions in freshly isolated neurons and microglia from the injured tissue. There were marked and diverse, sustained neurobehavioral changes in injured mice. Compared to sham

SUBMITTER: Ritzel RM 

PROVIDER: S-EPMC7155942 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature