IL-17A inhibitions of indole alkaloids from traditional Chinese medicine Qing Dai.

ABSTRACT:

Ethnopharmacological relevance

Qing Dai, a famous traditional Chinese medicine (TCM), is prepared by a traditional fermentation process with the aerial part of Strobilanthes cusia. Currently, this TCM could treat various clinical inflammatory diseases, such as ulcerative colitis and psoriasis, however, the bioactive components of Qing Dai are unknown clearly.

Aim of the study

To isolate and identify the anti-IL-17A components of Qing Dai.

Materials and methods

Silica, RP-18 gels, and size exclusion resin were used for column chromatography to isolate the pure compounds. The structures of isolates were elucidated by NMR, MS, UV, IR spectra, and optical rotation. IL-17A protein and gene expressions were also evaluated in the Th17 cell model and luciferase reporter assay

SUBMITTER: Lee CL 

PROVIDER: S-EPMC7156250 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature