Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue.

ABSTRACT: Although various production methods for 3D vascularised tissues have been developed, constructing capillary-like structures branching from perfusable large channels remains difficult. This study describes a method to fabricate tube-shaped 3D liver-like tissue (tubular liver tissue) with large channels and capillary-like structures using a perfusion device. The perfusion device functions as an interface between the tissue and an external pump, as it has connectors equipped with anchors that hold the tissue in response to its shrinkage, which is accompanied by the self-organisation of capillary-like structures. Histological analysis revealed that perfusion via the large channel induced capillary formation around the channel and maintained proper tissue functions. Accompanied by structural ex

SUBMITTER: Mori N 

PROVIDER: S-EPMC7156376 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature