Effect of UVC Irradiation on the Oxidation of Histidine in Monoclonal Antibodies.

ABSTRACT: We oxidized histidine residues in monoclonal antibody drugs of immunoglobulin gamma 1 (IgG1) using ultraviolet C irradiation (UVC: 200-280 nm), which is known to be potent for sterilization or disinfection. Among the reaction products, we identified asparagine and aspartic acid by mass spectrometry. In the photo-induced oxidation of histidine in angiotensin II, ¹⁸O atoms from H₂¹⁸O in the solvent were incorporated only into aspartic acid but not into asparagine. This suggests that UVC irradiation generates singlet oxygen and induces [2 + 2] cycloaddition to form a dioxetane involving the imidazole C^γ - C^δ2 bond of histidine, followed by ring-opening in the manner of further photo-induced retro [2 + 2] cycloaddition. This yields an equi

SUBMITTER: Miyahara Y 

PROVIDER: S-EPMC7156388 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature