Analysis of Mrgprb2 Receptor-Evoked Ca ²⁺ Signaling in Bone Marrow Derived (BMMC) and Peritoneal (PMC) Mast Cells of TRPC-Deficient Mice.

ABSTRACT: Mast cells are a heterogeneous group of immune cells. The simplest and commonly accepted classification divides them in two groups according to their protease content. We have compared the action of diverse secretagogues on bone marrow derived (BMMC) and peritoneal (PMC) mast cells which represent classical models of mucosal and connective tissue type mast cells in mice. Whereas, antigen stimulation of the FcεRI receptors was similarly effective in triggering elevations of free intracellular Ca²⁺ concentration ([Ca²⁺]_i) in both BMMC and PMC, robust [Ca²⁺]_i rise following Endothelin-1 stimulation was observed only in a fraction of BMMC. Leukotriene C4 activating cysteinyl leukotriene type I receptors failed to evoke [Ca²⁺]

SUBMITTER: Tsvilovskyy V 

PROVIDER: S-EPMC7156601 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature