Ribosome profiling at isoform level reveals evolutionary conserved impacts of differential splicing on the proteome.

ABSTRACT: The differential production of transcript isoforms from gene loci is a key cellular mechanism. Yet, its impact in protein production remains an open question. Here, we describe ORQAS (ORF quantification pipeline for alternative splicing), a pipeline for the translation quantification of individual transcript isoforms using ribosome-protected mRNA fragments (ribosome profiling). We find evidence of translation for 40-50% of the expressed isoforms in human and mouse, with 53% of the expressed genes having more than one translated isoform in human, and 33% in mouse. Differential splicing analysis revealed that about 40% of the splicing changes at RNA level are concordant with changes in translation. Furthermore, orthologous cassette exons between human and mouse preserve the directionality of

SUBMITTER: Reixachs-Sole M 

PROVIDER: S-EPMC7156646 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature