Cardiolipin is required in vivo for the stability of bacterial translocon and optimal membrane protein translocation and insertion.

ABSTRACT: Translocation of preproteins across the Escherichia coli inner membrane requires anionic lipids by virtue of their negative head-group charge either in vivo or in situ. However, available results do not differentiate between the roles of monoanionic phosphatidylglycerol and dianionic cardiolipin (CL) in this essential membrane-related process. To define in vivo the molecular steps affected by the absence of CL in protein translocation and insertion, we analyzed translocon activity, SecYEG stability and its interaction with SecA in an E. coli mutant devoid of CL. Although no growth defects were observed, co- and post-translational translocation of α-helical proteins across inner membrane and the assembly of outer membrane β-barrel precursors were severely compromised in CL-lacking cells. Co

SUBMITTER: Ryabichko S 

PROVIDER: S-EPMC7156725 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature