Identification and local manipulation of bone marrow vasculature during intravital imaging.

ABSTRACT: Physiological regulation of blood flow in bone marrow is important to maintain oxygen and glucose supplies but also the physiological hypoxic state of the hematopoietic stem cell (HSC) niche. However, regulatory mechanisms underlying microcirculation in the bone marrow (BM) niche remain unclear. Here, we identify vessels functioning in control of blood flow in bone marrow and assess their contractility. To evaluate contractile potential of Alexa Fluor 633 (AF633; an arterial marker)-positive vessels, we performed immunohistochemistry for α-smooth muscle actin (α-SMA) and found it expressed around AF633⁺ vessels in the femoral and calvarial marrow. To validate AF633⁺ vessel contractility, we developed a simple system to locally administer vasoactive agents that penetra

SUBMITTER: Morikawa T 

PROVIDER: S-EPMC7156750 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature