Discovery of inner centromere protein-derived small peptides for cancer imaging and treatment targeting survivin.

ABSTRACT: Survivin belongs to the inhibitor of apoptosis protein family, which is consistently overexpressed in most cancer cells but rarely expressed in normal adult tissues. Therefore, the detection and inhibition of survivin are regarded as attractive strategies for cancer-specific treatment. In this study, we designed and synthesized 7-19 residues of inner centromere protein (INCENP)-derived small peptides (INC peptides) as novel survivin-targeting agents. The INC peptides showed binding affinity for the human survivin protein (K_d  = 91.4-255 nmol L^-1 ); INC_16-22 , which contains residues 16-22 of INCENP, showed the highest affinity (91.4 nmol L^-1 ). Confocal fluorescence imaging showed consistent colocalization of FITC-INC_16-22 and survivin

SUBMITTER: Fuchigami T 

PROVIDER: S-EPMC7156834 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature