Multiple links between 5-methylcytosine content of mRNA and translation.

ABSTRACT:

Background

5-Methylcytosine (m⁵C) is a prevalent base modification in tRNA and rRNA but it also occurs more broadly in the transcriptome, including in mRNA, where it serves incompletely understood molecular functions. In pursuit of potential links of m⁵C with mRNA translation, we performed polysome profiling of human HeLa cell lysates and subjected RNA from resultant fractions to efficient bisulfite conversion followed by RNA sequencing (bsRNA-seq). Bioinformatic filters for rigorous site calling were devised to reduce technical noise.

Results

We obtained ~ 1000 candidate m⁵C sites in the wider transcriptome, most of which were found in mRNA. Multiple novel sites were validated by amplicon-specific bsRNA-seq in independent samples of either h

SUBMITTER: Schumann U 

PROVIDER: S-EPMC7158060 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature