Automation of Spatial Transcriptomics library preparation to enable rapid and robust insights into spatial organization of tissues.

ABSTRACT:

Background

Interest in studying the spatial distribution of gene expression in tissues is rapidly increasing. Spatial Transcriptomics is a novel sequencing-based technology that generates high-throughput information on the distribution, heterogeneity and co-expression of cells in tissues. Unfortunately, manual preparation of high-quality sequencing libraries is time-consuming and subject to technical variability due to human error during manual pipetting, which results in sample swapping and the accidental introduction of batch effects. All these factors complicate the production and interpretation of biological datasets.

Results

We have integrated an Agilent Bravo Automated Liquid Handling Platform into the Spatial Transcriptomics workflow. Compared to the previously report

SUBMITTER: Berglund E 

PROVIDER: S-EPMC7158132 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature