Native internally calibrated chromatin immunoprecipitation for quantitative studies of histone post-translational modifications.

ABSTRACT: Chromatin immunoprecipitation coupled to next-generation sequencing (ChIP-seq) has served as the central method for the study of histone modifications for the past decade. In ChIP-seq analyses, antibodies selectively capture nucleosomes bearing a modification of interest and the associated DNA is then mapped to the genome to determine the distribution of the mark. This approach has several important drawbacks: (i) ChIP interpretation necessitates the assumption of perfect antibody specificity, despite growing evidence that this is often not the case. (ii) Common methods for evaluating antibody specificity in other formats have little or no bearing on specificity within a ChIP experiment. (iii) Uncalibrated ChIP is reported as relative enrichment, which is biologically meaningless outside t

SUBMITTER: Grzybowski AT 

PROVIDER: S-EPMC7158589 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature