Systematic approach for assessing whether undeletable chromosomal regions in Saccharomyces cerevisiae are required for cell viability.

ABSTRACT: Previously, we identified 49 undeletable chromosomal regions harboring only non-essential genes in the genome of Saccharomyces cerevisiae. We proposed that there might be unknown synthetic lethal combinations of genes present in such undeletable regions of the genome. In this study, we chose four of the smallest undeletable chromosomal regions among the 49 and performed extensive further analyses to narrow down the gene-pairs responsible for lethality by replacing sub-regions in various combinations with a DNA module comprising the CgLEU2 marker. Although the methodology was different from previous study, interestingly the results revealed that not only the sub-regions but also the entire region was replaceable. To solve the apparent discrepancy between previous and present results, we fur

SUBMITTER: Hassan N 

PROVIDER: S-EPMC7158983 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature