Detection of bovine viral diarrhea virus genotype 1 in aerosol by a real time RT-PCR assay.

ABSTRACT:

Background

As a pestivirus of the Flaviviridae family, bovine viral diarrhea virus (BVDV), has imposed a large burden on animal husbandry worldwide, and such virus can be transmitted mainly through direct contact with other infected animals and probably via aerosols. In the present study, we aimed to develop a real-time RT-PCR method for detection of BVDV-1 in aerosol samples.

Methods

A pair of primers specific for highly conserved regions of the BVDV-1 5'-UTR was designed. The standard curve and sensitivity of the developed assay were assessed based on 10-fold serial dilutions of RNA molecular standard. The specificity of the assay was evaluated with other pestiviruses and infectious bovine viruses. The clinical performance was examined by testing 169 aerosol samples.

Re

SUBMITTER: Hou P 

PROVIDER: S-EPMC7159024 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature