Qualitative and quantitative ultrastructural analysis of the membrane rearrangements induced by coronavirus.

ABSTRACT: Coronaviruses (CoV) are enveloped positive-strand RNA viruses that induce different membrane rearrangements in infected cells in order to efficiently replicate and assemble. The origin, the protein composition and the function of these structures are not well established. To shed further light on these structures, we have performed a time-course experiment in which the mouse hepatitis virus (MHV)-induced membrane rearrangements were examined qualitatively and quantitatively by (immuno)-electron microscopy. With our approach we were able to confirm the appearance of 6, previously reported, membranous structures during the course of a complete infection cycle. These structures include the well-characterized double-membrane vesicles (DMVs), convoluted membranes (CMs) and virions but also the

SUBMITTER: Ulasli M 

PROVIDER: S-EPMC7159092 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature