Effects of amotosalen treatment on human platelet lysate bioactivity: A proof-of-concept study.

ABSTRACT: BACKGROUND:Clinical application of mesenchymal stromal cells (MSCs) usually requires an in vitro expansion step to reach clinically relevant numbers. In vitro cell expansion necessitates supplementation of basal mammalian cell culture medium with growth factors. To avoid using supplements containing animal substances, human platelet lysates (hPL) produced from expired and pathogen inactivated platelet concentrates can be used in place of fetal bovine serum. However, globally, most transfusion units are currently not pathogen inactivated. As blood banks are the sole source of platelet concentrates for hPL production, it is important to ensure product safety and standardized production methods. In this proof-of-concept study we assessed the feasibility of producing hPL from expired platelet

SUBMITTER: Christensen C 

PROVIDER: S-EPMC7159197 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature