A 3-step Mechanical Digestion Method to Harvest Adipose-derived Stromal Vascular Fraction.

ABSTRACT:

Background

Adipose stromal vascular fraction (SVF) isolation with enzymatic digestion is the gold standard, but is expensive, having practical and legal concerns. The alternative mechanical SVF isolation methods provide lower cell yields as they employ either centrifugation, emulsification, or digestion steps alone. We combined mechanical processing with buffer incubation and centrifugation steps into an isolation method called "mechanical digestion" and compared the cell yields with that of enzymatic digestion.

Methods

A total of 40-mL lipoaspirate was harvested from 35 women undergoing liposuction and was submitted to conventional enzymatic digestion for SVF isolation or mechanical digestion using a closed unit harnessing 3 ports with blades, followed by buffer incubation

SUBMITTER: Tiryaki T 

PROVIDER: S-EPMC7159941 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature