Challenges in the annotation of pseudoenzymes in databases: the UniProtKB approach.

ABSTRACT: The universal protein knowledgebase (UniProtKB) collects and centralises functional information on proteins across a wide range of species. In addition to the functional information added to all protein entries, for enzymes, which represent 20-40% of most proteomes, UniProtKB provides additional information about Enzyme Commission classification, catalytic activity, cofactors, enzyme regulation, kinetics and pathways, all based on critical assessment of published experimental data. Computer-based analysis and structural data are used to enrich the annotation of the sequence through the identification of active sites and binding sites. While the annotation of enzymes is well-defined, the curation of pseudoenzymes in UniProtKB has highlighted some challenges: how to identify them, how to ass

SUBMITTER: Zaru R 

PROVIDER: S-EPMC7160037 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature