Altered pathways in methylome and transcriptome longitudinal analysis of normal weight and bariatric surgery women.

ABSTRACT: DNA methylation could provide a link between environmental, genetic factors and weight control and can modify gene expression pattern. This study aimed to identify genes, which are differentially expressed and methylated depending on adiposity state by evaluating normal weight women and obese women before and after bariatric surgery (BS). We enrolled 24 normal weight (BMI: 22.5 ± 1.6 kg/m²) and 24 obese women (BMI: 43.3 ± 5.7 kg/m²) submitted to BS. Genome-wide methylation analysis was conducted using Infinium Human Methylation 450 BeadChip (threshold for significant CpG sites based on delta methylation level with a minimum value of 5%, a false discovery rate correction (FDR) of q < 0.05 was applied). Expression levels were measured using HumanHT-12v4 Expression BeadC

SUBMITTER: Nicoletti CF 

PROVIDER: S-EPMC7160100 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature