Resveratrol attenuates hypoxia-induced neuronal cell death, inflammation and mitochondrial oxidative stress by modulation of TRPM2 channel.

ABSTRACT: Hypoxia (HYPX) induced-overload Ca²⁺ entry results in increase of mitochondrial oxidative stress, inflammation and apoptosis in several neurons. Ca²⁺ permeable TRPM2 channel was gated by ADP-ribose (ADPR) and reactive oxygen species (ROS), although its activity was modulated in HYPX-exposed neurons by resveratrol (RSV). The aim of this study was to evaluate if a therapy of RSV can modulate the effect of HYPX in the TRPM2 expressing SH-SY5Y neuronal and HEK293 (no expression of TRPM2) cell lines. The SH-SY5Y and HEK293 cells were divided into four groups as control, RSV (50 μM and 24 hours), and HYPX and RSV + HYPX. For induction of HYPX in the cells, CoCl₂ (200 μM and 24 hours) incubation was used. HYPX-induced intracellular Ca²⁺ responses to TRP

SUBMITTER: Akyuva Y 

PROVIDER: S-EPMC7160154 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature