Programmable Synthetic Protein Circuits for the Identification and Suppression of Hepatocellular Carcinoma.

ABSTRACT: Precisely identifying and killing tumor cells are diligent pursuits in oncotherapy. Synthesized gene circuits have emerged as an intelligent weapon to solve these problems. Gene circuits based on post-transcriptional regulation enable a faster response than systems based on transcriptional regulation, which requires transcription and translation, showing superior safety. In this study, synthetic-promoter-free gene circuits possessing two control layers were constructed to improve the specific recognition of tumor cells. Using split-TEV, we designed and verified the basic control layer of protein-protein interaction (PPI) sensing. Another orthogonal control layer was built to sense specific proteins. Two layers were integrated to generate gene circuits sensing both PPI and specific proteins

SUBMITTER: Han X 

PROVIDER: S-EPMC7160531 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature