Characterization and Immunogenicity of HIV Envelope gp140 Zera^® Tagged Antigens.

ABSTRACT: HIV-1 envelope glycoprotein (Env) remains the most relevant target for the elicitation of functional antibodies to HIV by vaccination. However, soluble Env antigens often do not elicit the desired immune responses. Delivering subunit antigens on particulate nanoparticles is an established approach to improve their immunogenicity. In this study the sequence encoding Zera^®, a proline-rich domain derived from the γ-zein storage protein, was fused to either the C- or N-terminus of the superinfecting HIV-1 CAP256 gp140 envelope: Zera^® generally induces the formation of protein bodies (PBs), which can significantly improve both the immunogenicity and yields of the partner protein. The expression of gp140-Zera^® and Zera^®-gp140 (N- and C-terminal fusions

SUBMITTER: Ximba P 

PROVIDER: S-EPMC7160593 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature