Traction microscopy with integrated microfluidics: responses of the multi-cellular island to gradients of HGF.

ABSTRACT: Collective cellular migration plays a central role in development, regeneration, and metastasis. In these processes, mechanical interactions between cells are fundamental but measurement of these interactions is often hampered by technical limitations. To overcome some of these limitations, here we describe a system that integrates microfluidics with traction microscopy (TM). Using this system we can measure simultaneously, and in real time, migration speeds, tractions, and intercellular tension throughout an island of confluent Madin-Darby canine kidney (MDCK) cells. The cell island is exposed to hepatocyte growth factor (HGF) at a controlled gradient of concentrations; HGF is known to elicit epithelial-to-mesenchymal transition (EMT) and cell scattering. As expected, the rate of expansio

SUBMITTER: Jang H 

PROVIDER: S-EPMC7161022 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature