Emergence of carbapenem-resistant and colistin-susceptible Enterobacter cloacae complex co-harboring bla_IMP-1 and mcr-9 in Japan.

ABSTRACT:

Background

The spread of Enterobacteriaceae producing both carbapenemases and Mcr, encoded by plasmid-mediated colistin resistance genes, has become a serious public health problem worldwide. This study describes three clinical isolates of Enterobacter cloacae complex co-harboring bla_IMP-1 and mcr-9 that were resistant to carbapenem but susceptible to colistin.

Methods

Thirty-two clinical isolates of E. cloacae complex non-susceptible to carbapenems were obtained from patients at 14 hospitals in Japan. Their minimum inhibitory concentrations (MICs) were determined by broth microdilution methods and E-tests. Their entire genomes were sequenced by MiSeq and MinION methods. Multilocus sequence types were determined and a phylogenetic tree constructed by single nucleo

SUBMITTER: Kananizadeh P 

PROVIDER: S-EPMC7161257 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature