Axon microdissection and transcriptome profiling reveals the in vivo RNA content of fully differentiated myelinated motor axons.

ABSTRACT: Axonal protein synthesis has been shown to play a role in developmental and regenerative growth, as well as in the maintenance of the axoplasm in a steady state. Recent studies have begun to identify the mRNAs localized in axons, which could be translated locally under different conditions. Despite that by now hundreds or thousands of mRNAs have been shown to be localized into the axonal compartment of cultured neurons in vitro, knowledge of which mRNAs are localized in mature myelinated axons is quite limited. With the purpose of characterizing the transcriptome of mature myelinated motor axons of peripheral nervous systems, we modified the axon microdissection method devised by Koenig, enabling the isolation of the axoplasm RNA to perform RNA-seq analysis. The transcriptome analysis indi

SUBMITTER: Farias J 

PROVIDER: S-EPMC7161357 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature