Melatonin up-regulates bone marrow mesenchymal stem cells osteogenic action but suppresses their mediated osteoclastogenesis via MT₂ -inactivated NF-κB pathway.

ABSTRACT:

Background and purpose

Melatonin is a neurohormone involved in bone homeostasis. Melatonin directs bone remodelling and the role of bone marrow mesenchymal stem cells (BMMSCs) in the regulating melatonin-mediated bone formation-resorption balance remains undefined.

Experimental approach

Osteoporosis models were established and bone tissue and serum were collected to test the effects of melatonin on bone homeostasis. Melatonin receptors were knocked down, the NF-κB signalling pathway and receptor activator of NF-κB ligand (RANKL) expression were investigated. Communication between bone marrow mesenchymal stem cells and osteoclasts was detected with direct-contact or indirect-contact system.

Key results

Bone loss and microstructure disorder in mice were reversed after m

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC7161576 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature