Ribonucleic Acid Sequence Characterization by Negative Electron Transfer Dissociation Mass Spectrometry.

ABSTRACT: Modified oligonucleotides represent a promising avenue for drug development, with small interfering RNAs (siRNA) and microRNAs gaining traction in the therapeutic market. Mass spectrometry (MS)-based analysis offers many benefits for characterizing modified nucleic acids. Negative electron transfer dissociation (NETD) has proven valuable in sequencing oligonucleotide anions, particularly because it can retain modifications while generating sequence-informative fragments. We show that NETD can be successfully implemented on a widely available quadrupole-Orbitrap-linear ion trap mass spectrometer that uses a front-end glow discharge source to generate radical fluoranthene reagent cations. We characterize both unmodified and modified ribonucleic acids and present the first application of acti

SUBMITTER: Peters-Clarke TM 

PROVIDER: S-EPMC7161943 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature