Inhibition of herpes simplex virus type 1 by small interfering RNA.

ABSTRACT:

Background

RNA interference, a conserved mechanism in which a sequence-specific gene-silencing process is mediated by small interfering RNA (siRNA), is a promising method of gene therapy in treating a variety of viral diseases.

Aim

To investigate the antiviral effects of siRNA on herpes simplex virus type 1 (HSV-1) replication in Vero cells.

Methods

The antiviral effects of siRNA duplexes targeting the VP16 and DNA polymerase genes of HSV-1 were evaluated by yield-reduction and plaque-reduction assays. The effect of siRNA on the expression of target genes was measured by real-time quantitative reverse transcription PCR.

Results

Two siRNA duplexes (siRNA-1, targeting VP16, and siRNA-4, targeting DNA polymerase), were found to be highly effective in inhibiting HS

SUBMITTER: Zhang YQ 

PROVIDER: S-EPMC7162058 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature