Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs.

ABSTRACT:

Background/objectives

Vaccination is the most important tool for controlling brucellosis, but currently there is no vaccine available for canine brucellosis, which is a zoonotic disease of worldwide distribution caused by Brucella canis. This study aimed to evaluate protection and immune response induced by Brucella ovis ΔabcBA (BoΔabcBA) encapsulated with alginate against the challenge with Brucella canis in mice and to assess the safety of this strain for dogs.

Methods

Intracellular growth of the vaccine strain BoΔabcBA was assessed in canine and ovine macrophages. Protection induced by BoΔabcBA against virulent Brucella canis was evaluated in the mouse model. Safety of the vaccine strain BoΔabcBA was assessed in experimentally inoculated dogs.

Results

Wild type B.

SUBMITTER: Eckstein C 

PROVIDER: S-EPMC7162469 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature