BOLD and GenBank revisited - Do identification errors arise in the lab or in the sequence libraries?

ABSTRACT: Applications of biological knowledge, such as forensics, often require the determination of biological materials to a species level. As such, DNA-based approaches to identification, particularly DNA barcoding, are attracting increased interest. The capacity of DNA barcodes to assign newly encountered specimens to a species relies upon access to informatics platforms, such as BOLD and GenBank, which host libraries of reference sequences and support the comparison of new sequences to them. As parameterization of these libraries expands, DNA barcoding has the potential to make valuable contributions in diverse applied contexts. However, a recent publication called for caution after finding that both platforms performed poorly in identifying specimens of 17 common insect species. This study fo

SUBMITTER: Pentinsaari M 

PROVIDER: S-EPMC7162515 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature