Urban airborne particle exposure impairs human lung and blood Mycobacterium tuberculosis immunity.

ABSTRACT:

Rationale

Associations between urban (outdoor) airborne particulate matter (PM) exposure and TB and potential biological mechanisms are poorly explored.

Objectives

To examine whether in vivo exposure to urban outdoor PM in Mexico City and in vitro exposure to urban outdoor PM_2.5 (< 2.5 µm median aerodynamic diameter) alters human host immune cell responses to Mycobacterium tuberculosis.

Methods

Cellular toxicity (flow cytometry, proliferation assay (MTS assay)), M. tuberculosis and PM_2.5 phagocytosis (microscopy), cytokine-producing cells (Enzyme-linked immune absorbent spot (ELISPOT)), and signalling pathway markers (western blot) were examined in bronchoalveolar cells (BAC) and peripheral blood mononuclear cells (PBMC) from health

SUBMITTER: Torres M 

PROVIDER: S-EPMC7162557 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature