Long non-coding RNA LOC100133669 promotes cell proliferation in oesophageal squamous cell carcinoma.

ABSTRACT:

Objectives

LOC100133669 is a lncRNA whose function during tumorigenesis remains unclear now. Thus, we aimed to explore its clinical significance and function in oesophageal squamous cell carcinoma (ESCC).

Materials and methods

ISH was used to detect LOC100133669 expression in ESCC tissues. The full-length LOC100133669 was identified by using RACE assay. Subcellular distribution of LOC100133669 was examined by nuclear/cytoplasmic RNA fractionation and qPCR. The role of LOC100133669 in ESCC cell growth was determined by colony formation, MTT and flow cytometry experiments in vitro, as well as xenograft tumour experiment in vivo. RNA pull-down assay was performed to find LOC100133669-interacted protein, which was further examined by RIP, IP, Western blot and rescue experiments.

SUBMITTER: Guan Z 

PROVIDER: S-EPMC7162797 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature