Derivation of porcine extraembryonic endoderm-like cells from blastocysts.

ABSTRACT:

Objectives

Extraembryonic endoderm (XEN) cells are isolated from primitive endoderm (PrE) of blastocysts. Just like PrE, XEN cells have the ability to differentiate into parietal endoderm (PE) and visceral endoderm (VE), and therefore, they are useful tools for studying mechanisms of PrE cells development and differentiation. Pig is an ideal model for studying human cardiovascular and metabolic diseases and a potential organ source for allotransplantation, while no XEN cell has been obtained from porcine embryos.

Materials and methods

Using a serum-free culture system, we directly derived porcine extraembryonic endoderm-like cells (pXEN-like cells) from day 6-7 blastocysts, which could maintain self-renewal for at least 30 passages.

Results

The pXEN-like cells resembl

SUBMITTER: Li Y 

PROVIDER: S-EPMC7162807 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature