Airway basal cell injury after acute diacetyl (2,3-butanedione) vapor exposure.

ABSTRACT:

Rationale

Diacetyl (DA; 2,3-butanedione) is a chemical found commonly in foods and e-cigarettes. When inhaled, DA causes epithelial injury, though the mechanism of repair remain poorly understood. The objective of this study was to evaluate airway basal cell repair after DA vapor exposure.

Methods

Primary human bronchial epithelial cells were exposed to DA or PBS for 1 h. Lactate dehydrogenase, cleaved caspase 3/7 and trans-epithelial electrical resistance were measured prior to and following exposure. Exposed cultures were analyzed for the airway basal cell markers keratin 5 and p63 as well as ubiquitin and proteasome activity. Cultures were also treated with a proteasome inhibitor (MG132).

Results

DA vapor exposure caused a transient decrease in trans-epithelial ele

SUBMITTER: McGraw MD 

PROVIDER: S-EPMC7164319 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature