Key role of MIF-related neuroinflammation in neurodegeneration and cognitive impairment in Alzheimer's disease.

ABSTRACT:

Background

Macrophage Migration Inhibitory Factor (MIF) is a potent proinflammatory cytokine that promotes the production of other immune mediators. MIF is produced by most cell types in the brain including microglia, astrocytes and neurons. Enhanced expression of MIF might contribute to the persistent activation of glial, chronic neuroinflammation and neurodegeneration. Here, we investigated the effect of MIF on inflammatory markers and spatial learning in a mouse model of sporadic AD and on tau pathology in AD patients.

Methods

We examined the effects of MIF deficiency and pharmacological MIF inhibition in vitro and in vivo. In vitro, quantitative PCR and ELISA were used to assess cytokine production of STZ-treated glial cells. In vivo, C57BL/6 mice were subjected to intra

SUBMITTER: Nasiri E 

PROVIDER: S-EPMC7164357 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature