Characterisation of extraembryonic endoderm-like cells from mouse embryonic fibroblasts induced using chemicals alone.

ABSTRACT:

Background

The development of somatic reprogramming, especially purely chemical reprogramming, has significantly advanced biological research. And chemical-induced extraembryonic endoderm-like (ciXEN) cells have been confirmed to be an indispensable intermediate stage of chemical reprogramming. They resemble extraembryonic endoderm (XEN) cells in terms of transcriptome, reprogramming potential, and developmental ability in vivo. However, the other characteristics of ciXEN cells and the effects of chemicals and bFGF on the in vitro culture of ciXEN cells have not been systematically reported.

Methods

Chemicals and bFGF in combination with Matrigel were used to induce the generation of ciXEN cells derived from mouse embryonic fibroblasts (MEFs). RNA sequencing was utilised to

SUBMITTER: He X 

PROVIDER: S-EPMC7164364 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature