Ontology highlight
ABSTRACT:
SUBMITTER: Coelho S
PROVIDER: S-EPMC7164934 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Coelho Simao S Baek Jongho J Graus Matthew S MS Halstead James M JM Nicovich Philip R PR Feher Kristen K Gandhi Hetvi H Gooding J Justin JJ Gaus Katharina K
Science advances 20200417 16
Single-molecule localization microscopy (SMLM) has the potential to quantify the diversity in spatial arrangements of molecules in intact cells. However, this requires that the single-molecule emitters are localized with ultrahigh precision irrespective of the sample format and the length of the data acquisition. We advance SMLM to enable direct distance measurements between molecules in intact cells on the scale between 1 and 20 nm. Our actively stabilized microscope combines three-dimensional ...[more]